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Vanhu pasi rese vanoshandisa zvinwiwa zvesimba kuti vavandudze kutarisa kwavo nekubudirira.Imwe yenzira dzakanakisisa dzekuongorora zvinwiwa izvi ndeye capillary electrophoresis.Ichi chinyorwa chinoongorora kugona uye kukosha mukuenzanisa nedzimwe nzira dzakadai semvura chromatography.
Zvinwiwa zvakawanda zvesimba zvinogadzirwa kubva kune caffeine-rich compounds, kusanganisira caffeine uye glutamate.Caffeine is stimulant alkaloid inowanikwa mumhando dzinopfuura makumi matanhatu nenhatu dzezvirimwa pasi rose.Yakachena caffeine inovava, isina kunaka, yakachena yakasimba.Molecular huremu hwecaffeine 194.19 g, kunyunguduka 2360°C.Caffeine ine hydrophilic pakamuri tembiricha ine huwandu hwakanyanya hwe21.7 g/l nekuda kwekuita kwayo kuri pakati nepakati.
Zvinwiwa zvakapfava masisitimu akaomarara ane akawanda akasiyana masanganiswa, ese ari inorganic uye organic.Kupatsanura macheki kwakakosha kuona uye kuongorora mamwe marudzi akasiyana ecaffeine nemabenzoates.Nzira inonyanya kushandiswa kuongorora kupatsanurwa kwecombinatorial ndeye liquid chromatography (LC).
Liquid chromatography inonzi inoshandiswa kusiyanisa pakati pehuwandu hwakawanda hwema organic molecules, kubva kune madiki mamorekuru huremu anosvibisa kusvika kune antimicrobial peptides.Kupindirana kwakasiyana pakati peanofamba uye akamira nhanho dzemamorekuru mune sampuli ari pasi pekuparadzaniswa kwechimvura chromatography.Iyo yakasimba chisungo, zviri nani iyo molecule inobata nzvimbo yayo.
Imwe nzira kune HPLC maitiro ndeyekupatsanurwa neyakamanikana yakaboora fused silica capillary electrophoresis, iyo inoshandisa munda wemagetsi kupatsanura makomisheni kubva kumapoka emakemikari akasiyana mune imwe sampuli.CE inogona kukamurwa kuita akati wandei nzira dzekuparadzanisa zvichienderana necapillaries uye ion inoshandiswa.
Iyo capillary electrophoresis nzira inobatsira zvikuru pakuongorora kwechikafu uye chinwiwa nekuda kwezvakanaka zvayo zveyakaderera sampuli uye reagent kushandiswa, pfupi yekuongorora nguva, yakaderera mutengo wekushandisa, yakakwirira resolution, yakakwirira yekubvisa kunyatsoita, kureruka kwekuyedza uye nekukurumidza kuvandudza maitiro.
Iyo electrophoresis separation method inobva pakufamba kwakasiyana kwemakemikari ions musero re electrolytic pasi pechiito chemunda wemagetsi wakaiswa.Kuenzaniswa neyakaoma mvura chromatography michina, capillary electrophoresis midziyo iri nyore.Pombi yekubatanidza ine dhayamita yemukati me 25-100 m uye span ye 20-100 cm inobatanidza masero maviri e-buffer, mukati mawo magetsi epamusoro-voltage (0-30 kV) anopiwa kuburikidza nemakondukita uye dunhu rinoshanda re electrolysis rinotakurwa se carrier carrier.
Kazhinji, anode inoonekwa se capillary inlet uye cathode inoonekwa se capillary outlet.Chiyero chidiki chesample chinobaiwa hydraulically kana nemagetsi muanode side ye capillary.Motokari infusion inoitwa nekutsiva buffer reservoir nesample vial uye kushandisa magetsi ikozvino kwenguva yenguva kubvumira zvidimbu kuti zvifambe mu capillary.
Hydrostatic infusion inoburitsa sampuli zvichienderana nekudonha kwekudzvanywa pakati peinopinza nekubuda kwecapillary, uye huwandu hwesample yakabaiwa inotarwa nekudonha kwekumanikidza uye ukobvu hwepolymer matrix.Mushure mekunge sampu yatakurwa, chikamu chemuenzaniso chinoungana pakuvhurwa kwecapillary.
Kupatsanura zvimiro zvecapillary electrophoresis matekiniki anogona kuyerwa nenzira mbiri: kupatsanurwa kugadzirisa, maRs, uye kupatsanura kugona.Kugadziriswa kwevaongorori vaviri kunoratidza kuti vanogona sei kusiyanisa mumwe nemumwe.Iyo yakakura kukosha kweRs, iyo inonyanya kutaurwa iyo yepamusoro peak.Kuparadzaniswa kwekugadzirisa kunogadzirisa kupatsanurwa kwakanaka uye kunoongorora kana kugadzirisa munharaunda yekuedza kunogona kukonzera kupatsanurwa kwemisanganiswa.
Kuparadzanisa kushanda zvakanaka N inzvimbo yekufungidzira umo nhanho mbiri dziri mukuenzana, inomiririrwa nenhamba yemapaneru akasiyana, zvichienderana nekunaka kwekoramu uye mvura.
Chidzidzo chitsva chakabudiswa paInternational Conference on Agriculture and Sustainability chinangwa chekuongorora kukwanisa kwe capillary electrophoresis kuziva nitrogenous compounds uye ascorbic acid muzvinwiwa, pamwe chete nemigumisiro ye electrophoresis variables pahuwandu hwehuwandu hwemaitiro.
Zvakanakira capillary electrophoresis pamusoro pepamusoro kuita kwemvura chromatography inosanganisira yakaderera mutengo wekutsvagisa uye kuenderana kwezvakatipoteredza, pamwe nekuongororwa kweasymmetric organic acid kana base peaks.Capillary electrophoresis inopa kukwana kwakakwana kwekuzivikanwa kwemakemikari akaomeswa mumatrice akaomarara ane mamwe maparamita akakosha (kupararira kwekanyiwa mune inofamba buffer, kuve nechokwadi chehomogeneity yekuumbwa kwebuffer, kusimba kwekupisa kwezvikamu zvinopatsanura).
Muchidimbu, kunyangwe capillary electrophoresis ine akawanda mabhenefiti pamusoro peyakanyanya kuita yemvura chromatography, ine zvakare zvayakaipira senge nguva refu yekuongorora.Imwe tsvagiridzo inofanirwa kuitwa kutsvaga nzira dzekuvandudza nzira iyi.
Rashid, SA, Abdulla, SM, Najeeb, BH, Hamarashid, SH, & Abdulla, OA (2021). Rashid, SA, Abdulla, SM, Najeeb, BH, Hamarashid, SH, & Abdulla, OA (2021).Rashid, SA, Abdullah, SM, Najib, BH, Hamarasheed, SH, uye Abdullah, OA (2021).Rashid SA, Abdullah SM, Najib BH, Hamarasheed SH naAbdulla OA (2021).Kutsunga kwecaffeine uye sodium benzoate mune zvinotengeswa kunze uye zvemuno zvinwiwa zvesimba uchishandisa HPLC uye spectrophotometer.IOP Musangano Series: Pasi uye Environmental Sayenzi.Inowanikwa pa: https://iopscience.iop.org/article/10.1088/1755-1315/910/1/012129/meta.
ALVES, AC, MEINHART, AD, & FILHO, JT (2019). ALVES, AC, MEINHART, AD, & FILHO, JT (2019).ALVES, AS, MEINHART, AD, uye FILHO, JT (2019).ALVES, AS, MEINHART, AD uye FILHO, JT (2019).Kuvandudzwa kwenzira yekuongorora panguva imwe chete ye caffeine uye taurine musimba.Sayenzi Yezvokudya uye Tekinoroji.Inowanikwa pa: https://www.scielo.br/j/cta/a/7n534rVddj3rXJ89gzJLXvh/?lang=en
Tuma, Piotr, Frantisek Opekar, naPavel Dlouhy.(2021).Capillary uye microarray electrophoresis ine isingabatanidzi conductivity kutsunga kwechikafu uye chinwiwa kuongororwa.chikafu chemakemikari.131858. Inowanikwa pa: https://linkinghub.elsevier.com/retrieve/pii/S0308814621028648.
Khasanov, VV, Slizhov, YG, & Khasanov, VV (2013). Khasanov, VV, Slizhov, YG, & Khasanov, VV (2013).Khasanov VV, Slizhov Yu.G., Khasanov VV (2013).Khasanov VV, Slizhov Yu.G., Khasanov VV (2013).Kuongororwa kwezvinwiwa zvesimba ne capillary electrophoresis.Chinyorwa cheAnalytical Chemistry.Inowanikwa pa: https://link.springer.com/article/10.1134/S1061934813040047.
Fani, KK (207).Capillary kuongororwa kwezvidziviriro muzvinwiwa zvesimba.California Polytechnic State University, Pomona.Inowanikwa pa: https://scholarworks.calstate.edu/concern/theses/mc87ps371.
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Ibtisam akapedza kudzidza kubva kuIslamabad Institute of Space Technology aine bachelor's degree muaerospace engineering.Munguva yebasa rake redzidzo, anga achibatanidzwa muzvirongwa zvekutsvagisa zvakati wandei uye akabudirira kuronga akati wandei ekuwedzera-curricular zviitiko zvakaita seInternational World Space Week uye International Conference on Aerospace Engineering.Ibtisam akahwina makwikwi ekunyora echiRungu mumazuva ake emudzidzi uye agara achiratidza kufarira kutsvagisa, kunyora nekugadzirisa.Nguva pfupi apedza kudzidza, akabatana neAzoNetwork semubatsiri kuti avandudze hunyanzvi hwake.Ibtisam anofarira kufamba, kunyanya kumaruwa.Agara ari mutsigiri wemitambo uye aifarira kuona tennis, nhabvu nekiriketi.Akazvarwa muPakistan, Ibtisam anotarisira rimwe zuva kufamba pasirese.
Abbasi, Ibtisam.(Kubvumbi 4, 2022).Kuongororwa kwezvinwiwa zvesimba ne capillary electrophoresis.AZOM.Yakadzorerwa Gumiguru 13, 2022 kubva https://www.azom.com/article.aspx?ArticleID=21527.
Abbasi, Ibtisam."Kuongorora Kwesimba Drinks neCapillary Electrophoresis".AZOM.Gumiguru 13, 2022.Gumiguru 13, 2022.
Abbasi, Ibtisam."Kuongorora Kwesimba Drinks neCapillary Electrophoresis".AZOM.https://www.azom.com/article.aspx?ArticleID=21527.(Kubva Gumiguru 13, 2022).
Abbasi, Ibtisam.2022. Kuongororwa kwezvinwiwa zvesimba ne capillary electrophoresis.AZoM, yakasvika 13 Gumiguru 2022, https://www.azom.com/article.aspx?ArticleID=21527.
AZoM inotaura naDr. Chenge Jiao, Applications Research Fellow paThermo Fisher Scientific, nezvekushandisa gallium-isina yakatariswa ion danda kugadzirira-isina kukanganisa TEM samples.
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